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RNA begets RNA

RNA

Piece by piece. Researchers designed two RNA strands (blue and red) that could replicate indefinitely and evolve, showing how life could have arisen from these simple molecules.

Gerald Joyce, Scripps Institute
 As Science‘s essay on the origin of life on Earth points out, many researchers think RNA was central to early life. But one challenge has been to show that RNA could make copies of itself without help from another biological molecule–DNA or proteins, for example–and that it could do this for long enough to allow evolution to gain a foothold. “That’s no small feat,” says molecular biologist Joan Steitz of Yale University. Yet molecular biologist Gerald Joyce of the Scripps Research Institute in San Diego, California, reports success this week in Science Express.

When RNA replicates itself, it tends to make perfectly complementary copies that stick together like the jaws of a zipper. Once an RNA molecule has found its complementary match, it tends not to split off and make more copies. Joyce says his goal was to create an RNA molecule that was just unstable enough to keep replicating.

For almost a decade, his team played with different combinations of the four RNA nucleotides–A, U, G, C–to create an RNA that would replicate indefinitely. Nothing kept dividing. But finally, Joyce’s team hit on the idea of creating an RNA molecule that would replicate by copying and assembling whole chunks of molecule at a time rather than working letter by letter. They engineered two such RNA molecules, called E and E-prime, and dropped them into a solution with four strands of nucleotides, precursors that might have been available on the young Earth. Within an hour, the number of RNAs had doubled, and the molecules kept replicating until all the other nucleotides in the solution had been used up, the researchers report. The replicated RNAs aren’t perfect copies, and Joyce’s team has shown that some of these mutants can outcompete their parent RNAs, becoming more populous over time. Despite being simple–the RNAs are just 70 nucleotides long–they demonstrate primitive evolution, Joyce points out.

Although the work almost certainly doesn’t reflect what happened at the start of life, it models how self-replication and mutation might have arisen–a significant accomplishment, says Andy Ellington, a molecular biologist at the University of Texas, Austin.

About the author

Rachel Zelkowitz is a science writer in Washington, D.C.