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Posts tagged with "Analytical Chemistry"

  • Analytical Chemistry

    A New Way to Get Protein Crystal Structures

    There’s a report of a new technique to solve protein crystal structures on a much smaller scale than anyone’s done before. Here’s the paper: the team at the Howard Hughes Medical Institute has used cryo-electron microscopy to do electron diffraction on microcrystals of lysozyme protein. We present a method, ‘MicroED’, for stru… Read More
  • Analytical Chemistry

    Crystallography Without Crystallizing: An Update

    I wrote here about a very promising X-ray crystallography technique which produces structures of molecules that don’t even have to be crystalline. Soaking a test substance into a metal-organic-framework (MOF) lattice gave enough repeating order that x-ray diffraction was possible. The most startling part of the paper, other than the concept i… Read More
  • Analytical Chemistry

    Ligands From Nothing

    Well, nearly nothing. That’s the promise of a technique that’s been published by the Ernst lab from the University of Basel. They first wrote about this in 2010, in a paper looking for ligands to the myelin-associated glycoprotein (MAG). That doesn’t sound much like a traditional drug target, and so it isn’t. It’s part… Read More
  • Analytical Chemistry

    An HIV Structure Breakthrough? Or “Complete Rubbish”?

    Structural biology needs no introduction for people doing drug discovery. This wasn’t always so. Drugs were discovered back in the days when people used to argue about whether those “receptor” thingies were real objects (as opposed to useful conceptual shorthand), and before anyone had any idea of what an enzyme’s active sit… Read More
  • Analytical Chemistry

    The 3D Fragment Consortium

    Fragment-based screening comes up here fairly often (and if you’re interested in the field, you should also have Practical Fragments on your reading list). One of the complaints both inside and outside the fragment world is that there are a lot of primary hits that fall into flat/aromatic chemical space (I know that those two… Read More
  • Analytical Chemistry

    New Frontiers in Analytical Chemistry

    A reader sends this new literature citation along, from Organometallics. He directed my attention to the Supplementary Information file, page 12. And what do we find there? . . .Solvent was then removed to leave a yellow residue in the vial, the remaining clear, yellow solution was concentrated to a volume of about 1ml, and… Read More
  • Analytical Chemistry

    Touching Up the Spectra

    Organic chemists have been taking NMR spectra for quite a while now. Routine use came on in the 1960s, and higher-field instruments went from exotic big-ticket items in the 1970s to ordinary equipment in the 1980s. But NMR can tell you more about your sample than you wanted to know (good analytical techniques are annoying… Read More
  • Analytical Chemistry

    Picking Out Incorrect Natural Products

    Once in a while, you see people who’ve gone to the trouble of synthesizing a natural product, only to find that its structure had been incorrectly assigned. (Back in the days when structure elucidation was much harder, R. B. Woodward had this on his list of reasons to do total synthesis, although it wasn’t number… Read More
  • Analytical Chemistry

    GPCRs Are As Crazy As You Thought

    That’s my take-away from this paper, which takes a deep look at a reconstituted beta-adrenergic receptor via fluorine NMR. There are at least four distinct states (two inactive ones, the active one, and an intermediate), and the relationships between them are different with every type of ligand that comes in. Even the ones that look… Read More
  • Analytical Chemistry

    Watching DNA Polymerase Do Its Thing

    Single-molecule techniques are really the way to go if you’re trying to understand many types of biomolecules. But they’re really difficult to realize in practice (a complaint that should be kept in context, given that many of these experiments would have sounded like science fiction not all that long ago). Here’s an example of ju… Read More
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