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Posts tagged with "Drug Assays"

  • Drug Assays

    The Miracle Solvent

    For those who were wondering, my copper reactions the other day worked out just fine. They started out a beautiful blue (copper iodide and an amino acid in straight DMSO – if that’s not blue it’s maybe going to be green, and if it’s not either one you’ve done something wrong). Of course, the color… Read More
  • Animal Testing

    Whose Guess Is Better?

    I was having a discussion the other day about which therapeutic areas have the best predictive assays. That is, what diseases can you be reasonably sure of treating before your drug candidate gets into (costly) human trials? As we went on, things settled out roughly like this: Cardiovascular (circulatory): not so bad. We’ve got a… Read More
  • Analytical Chemistry

    Start Small, Start Right

    There’s an excellent paper in the most recent issue of Chemistry and Biology that illustrates some of what fragment-based drug discovery is all about. The authors (the van Aalten group at Dundee) are looking at a known inhibitor of the enzyme chitinase, a natural product called argifin. It’s an odd-looking thing – five amino acids… Read More
  • Biological News

    Getting Real With Real Cells

    I’ve been reading an interesting paper from JACS with the catchy title of “Optimization of Activity-Based Probes for Proteomic Profiling of Histone Deacetylase Complexes”. This is work from Benjamin Cravatt’s lab at Scripps, and it says something about me, I suppose, that I found that title of such interest that I immediately printed of… Read More
  • Animal Testing

    The Animal Testing Hierarchy

    I’ve had some questions about animal models and testing, so I thought I’d go over the general picture. As far as I can tell, my experience has been pretty representative. There are plenty of animal models used in my line of work, but some of them you see more than others. Mice and rats are… Read More
  • Analytical Chemistry

    These Fragments I Have Shored Against My Ruins

    There’s been a big trend the last few years in the industry to try to build our molecules up from much smaller pieces than usual. “Fragment-based” drug discovery is the subject of many conferences and review articles these days, and I’d guess that most decent-sized companies have some sort of fragment effort going on. (Recent… Read More
  • Drug Assays

    A Bad Assay: Better Than None?

    Man, do we ever have a lot of assays in this business. Almost every drug development project has a long list of them, arranged in what we call a screening cascade. You check to make sure that your new molecule hits your protein target, then you try it on one or more living cell lines. Read More
  • Biological News

    Let Us Now Turn To the Example of Yo’ Mama

    Now we open the sedate, learned pages of Nature Methods, a fine journal that specializes in new techniques in molecular and chemical biology. In the August issue, the correspondence section features. . .well, a testy response to a paper that appeared last year in Nature Methods. “Experimental challenge to a ‘rigorous’ BRET analysis of GPCR… Read More
  • Cancer

    The Current Cancer Long-Jump Record

    As I’ve mentioned before, advances in molecular biology have continued to make all sorts of brute-force approachs possible – things that would have been laughed at (or, more likely, not even proposed at all) a few years ago. Another recent example of this is a paper earlier this year in Nature from the group of… Read More
  • Drug Assays

    Travels In Numerica Deserta

    There’s a problem in the drug industry that people have recognized for some years, but we’re not that much closer to dealing with it than we were then. We keep coming up with these technologies and techniques which seem as if they might be able to help us with some of our nastiest problems – Read More